Order name solubl trn test number 3604710 revision date 03032009. Soluble transferrin receptor an overview sciencedirect. The yeast secretion apparatus was optimized by modulating expression temperature, the folding environment of the endoplasmic reticulum, and gene dosage. It imports iron by internalizing the transferrin iron complex through receptor mediated endocytosis. The uptake of iron by cells is mediated by a cellsurface transferrin receptor tfr. Pediatric reference intervals for soluble transferrin. Soluble transferrin receptorferritin index in the evaluation. Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum. The apotransferrinreceptor complex is then recycled to the cell surface with a return to neutral ph and the concomitant loss of affinity of apotransferrin for its receptor. Soluble transferrin receptor regional medical lab test. Soluble transferrin receptor discriminates iron deficiency anemia from anemia of chronic disease in leukemic and breast cancer patients.
The transferrin receptor is an integral membrane glycoprotein having a molecular weight of 190 kilodaltons kd. The most important determinant of serum stfr levels. The amount of transferrin receptor expressed on a cell is proportional to the cells need for iron. Where exposure to inflammation is high, soluble transferrin receptor stfr. Soluble transferrin receptor stfr is present in human plasma and is considered as a truncated form of the tissue receptor that exists as a transferrin receptor complex and therefore it reflects tissue iron deficiency8,9.
Production of soluble and active transferrin receptor. In most cases, the rash and pain subside within a few weeks, but some of those affected may have severe pain that lingers for. A soluble form of tfr stfr has been identified in animal 7 and human serum 8. These complexes bind to transferrin receptors found on the surface of cells and the iron is then transported into the cells.
The soluble transferrin receptor stfr has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia ida and anemia of chronic disease acd. Soluble transferrin receptor stfr is present in human plasma and is considered as a truncated form of the tissue receptor that exists as a transferrinreceptor complex and therefore it reflects tissue iron deficiency8,9. Soluble transferrin receptor stfr is a new marker of iron status and. Recombinant soluble transferrin receptor rstfr1st who. Serum soluble transferrin receptor concentration is an. Soluble transferrin receptor is elevated in association with hyperplastic erythropoiesis, for example, in patients with haemolytic anaemia or betathalassaemia. On monday, december 17, 2001, the clinical chemistry laboratory of the department of pathology will begin accepting specimens for the soluble transferrin receptor assay stfr. Recent studies showing an improved diagnosis of iron deficiency id with soluble transferrin receptor stfr and stfrferritin index did not take into account the agedependency of stfr and ferritin. Levels of this receptor are increased in irondeficiency anemia. Online conversion calculator for many types of measurement units in laboratory and medicine soluble transferrin receptor stfr, stfr, transferrin receptor, tfr, serum transferrin receptors. It imports iron by internalizing the transferriniron complex through receptormediated endocytosis. Pdf soluble transferrin receptor discriminates iron.
Soluble transferrin receptors and iron deficiency, a step. The concentration of soluble transferrin receptor stfr in serum is reported to be useful in the diagnosis of iron deficiency, especially for patients with concurrent chronic disease, where routine tests of iron status are compromised by the inflammatory condition. The prevalence of iron depletion, iron deficient erythropoiesis ide, and iron deficiency anemia ida was assessed in preschool inuit children using soluble transferrin receptor stfr and traditional indicators of iron status while disregarding or taking inflammation into account when defining sf cutoffs. After adding the product to the cart by clicking the button below you will see if there is a volume discount available. The developing red cells regulate their iron uptake by increasing the number of transferrin receptors on their surface, and the number of stfr in serum increases in parallel. Soluble transferrin receptor stfr conversion to nmoll. Soluble transferrin receptor definition of soluble.
Soluble transferrin receptors stfr are proteins found in blood that can become elevated in the presence of irondeficiency. The access soluble transferrin receptor stfr is considered the worlds first. The circulating stfr concentration is proportional to cellular expression of the membraneassociated tfr and increases with. A study cohort of 436 apparently healthy children was analyzed to establish reference. Proteolysis leads to the soluble form of the transferrin. The soluble transferrin receptor stfr concentration is increased in iron deficiency. Serum soluble transferrin receptor increases in iron deficiency and is usually unaffected by chronic disease states. Each of the monomers has an 85 kd cterminal component which can bind an ironladen transferrin molecule. Soluble transferrin receptor, human, antibody hytest web store.
Recombinant soluble transferrin receptor rstfr 1st who reference reagent type of standard. Transferrin receptor number, synthesis, and endocytosis during erythropoietininduced maturation of friend virusinfected erythroid cells received for publication, october 30, 1985 stephen t. Blood testing of the soluble transferrin receptor stfr is used as a measure of functional iron status and the. Soluble transferrin receptors and iron deficiency, a step beyond ferritin. Pdf the usefulness of soluble transferrin receptor stfr. Soluble transferrin receptor stfr test system product.
Soluble transferrin receptor stfr is a truncated form of the cellular transferrin receptor, without its transmembrane and cytoplasmic domains, and circulates bound to transferrin. The circulating stfr concentration is proportional to cellular expression of. The expression of transferrin receptor tfr has been identified in many malignant tumours. Targetspecific elisa kits are available from a variety of manufacturers. Soluble transferrin receptor arises from proteolysis of the intact protein on the cell surface, leading to monomers that can be measured in plasma and serum. Role of soluble transferrin receptor and transferrin. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia the reference range of transferrin receptor varies by sex in adults, as follows. Serum transferrin receptor levels for the assessment of iron status.
This study aim was to determine the diagnostic value of stfr and tfrf index and to combine these two markers in detecting ida in rh anaemic. When transferrin binds to iron, they form a complex. Soluble transferrin receptor, human, antibody hytest web. Pdf the usefulness of soluble transferrin receptor stfr in. The circulating stfr concentration is proportional to cellular expression of the membraneassociated. Soluble transferrin receptor elisa kits biocompare.
Transferrin receptor gene and protein expression and localization in human iugr and normal term placentas. Combining haematologic passport and use of models might be an. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia iron is an essential nutrient absorbed from the diet. Stfr is useful in distinguishing anemia of chronic disease from iron deficiency. Shingles, also called herpes zoster, is an infection caused by the varicella zoster virus vzv, the same virus that causes chickenpox. It is needed for the import of iron into the cell and is regulated in response to intracellular iron concentration. Biological variations and reference limits, clinical chemistry and laboratory medicine cclm on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds. Soluble transferrin receptors are proteins found in blood that can be elevated with iron deficiency. Pdf measurement of soluble transferrin receptor in serum of. The soluble transferrin receptor stfr test is primarily ordered to help distinguish between anemia that is caused by iron deficiency and anemia that is caused by inflammation or a chronic illness socalled anemia of chronic disease or inflammation. Transferrin receptor tfr is a carrier protein for transferrin. Soluble transferrin receptor for the evaluation of. New cutoff values for ferritin and soluble transferrin receptor for the. In recent years, soluble transferrin receptor stfr has been introduced as a sensitive, early and highly quantitative new marker of iron depletion, increasing in proportion to tissue iron deficit. The use of soluble transferrin receptor in the detection of rhuepo. Role of soluble transferrin receptor and transferrin receptor. To combine data, individual survey analyses, which accounted for the. Pediatric reference intervals for 28 chemistries and. It can cause nerve inflammation and intense pain, a reddened rash, and blisters that break open and crust over before slowly resolving.
Human soluble transferrin receptor,stfr elisa kit cusabio. It consists of two identical subunits linked by disulfide bridges. The transferrin receptor tfr is a homodimeric type ii membrane protein that mediates iron uptake into the cells. Elevated stfr values are found in anemia of chronic disease acd, iron deficiency anemia ida, polycythemia, hemolytic anemia, thalassemia, hereditary spherocytosis, sickle cell and megaloblastic anemia, myelodysplastic syndrome and vitamin b12 deficiency. In contrast, epidermal growth factor dissociates from its receptor and is directed to the lysosomes for proteolysis. The stfr reflects the iron demand of the body and is postulated as a regulator of iron homeostasis via binding to the hereditary hemochromatosis protein hfe.
The table also includes conversion formulas and the general method used for each analyte. Release of the soluble transferrin receptor is directly. Furthermore, the stfrlog ferritin index, combining ferritin and stfr values. Soluble transferrin receptor and ferritin concentrations were nephelometrically determined by n latex reagent sets bade behring according to the manufacturers instructions after storage of the centrifuged 2600g. Combining these markers into the tfrf index may prove to be a much better tool to detect iron deficiency in children in these high infection pressure areas. Soluble and cellassociated transferrin receptor in lung. Recombinant soluble transferrin receptor stfr hytest. Purchase human soluble transferrin receptor,stfr elisa kit. Use of multiple antibiotics in patients with cf is routine. Secreted scfv was purified using immobilized metal. Adjusting soluble transferrin receptor concentrations for. Utility of access soluble transferrin receptor stfr and stfrlog. The transferrin receptor is comprised of two identical subunits, each composed of 760 aminoacids with a molecular mass of 95 kdaltons.
Soluble transferrin receptor stfr test system product code. Moreover, there is a paucity of data on pediatric reference intervals for stfr and stfrferritin index. In general, to increase sensitivity and specificity, the measurement of serum soluble transferrin receptor should be performed in combination with other tests of iron status, including ferritin, tibc, and serum iron refer to. Cells can be treated with the ligand transferrin labeled with alexa fluor 546. Pdf we evaluated the usefulness of soluble transferrin receptor stfr and of the stfrlog ferritin. Soluble transferrin receptor a marker for iron deficiency. This gene encodes a singlepass type ii membrane protein, which is a member of the transferrin receptorlike family. The plasma transferrin receptor concentration is not increased with infection or inflammation, unlike plasma ferritin. Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apotransferrin is released again 41. Soluble tfr is a truncated monomer of tissue receptor, lacking its first 100 amino acids, which circulates in. Now also available on your iphone, ipad, and android. Approximately 600 specimens were analyzed in each of the two phases consisting of measuring 14 analytes each. In group 1, patients serum stfr levels correlated negatively with mean corpuscular volume.
Soluble transferrin receptor stfr conversion to nmoll, mgl, mgdl, mg100ml, mg%, gml. Transferrin receptor is a nonrtk constitutively recycling receptor and thus can be used as a control to assess rtk specificity of met trafficking. Is soluble transferrin receptor a good marker of iron. A new diagnostic assay for stfr is calibrated against natural plasma stfr, thus minimizing calibration discrepancies that result. Cellular uptake of iron occurs via receptormediated endocytosis of ligandoccupied transferrin receptor into specialized endosomes.
Erythroblast iron metabolism and serum soluble transferrin receptor values in the anemia of rheumatoid arthritis. Measurement of soluble transferrin receptor in serum of. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia iron is an essential nutrient absorbed from the diet. The human transferrin receptor tfr is shed by an integral metalloprotease releasing a soluble form stfr into serum. Feb 28, 2011 soluble transferrin receptor stfr is a truncated form of the cellular transferrin receptor, without its transmembrane and cytoplasmic domains, and circulates bound to transferrin. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia. Measurement of circulating levels of the receptor are very useful in the diagnosis of iron deficiency in patients who also have anemia. Thus, the concentration of stfr in plasma or serum is an indirect measure of total tfr. Transferrin receptors are cleaved from the surface of cells, enter the bloodstream, and become soluble transferrin receptors stfr. We assessed the possibility of using soluble transferrin receptor stfr as an indicator of doping with recombinant erythropoietin rhepo. A proteolytic product of the transferrin receptor circulates in plasma as soluble transferrin receptor stfr. Transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency.
Soluble transferrin receptor elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Transferrin receptor an overview sciencedirect topics. A potential molecular imaging marker for human cancer article pdf available in neoplasia 56. Recent studies have also suggested that stfr measurements are helpful when predicting the response to erythropoietin in patients with anaemia resultant from chronic renal failure. In lung cancer, lymphoma and breast cancer, it has been shown that the expression of tfr correlates with tumour differentiation, probably implying some prognostic value.
Pdf the concentration of soluble transferrin receptor stfr in serum is reported to be useful in the diagnosis of iron deficiency, especially for. Feb 12, 2014 transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. This study describes the soluble production, purification, and functional testing of an antitransferrin receptor singlechain antibody ox26 scfv using the yeast saccharomyces cerevisiae. This protein mediates cellular uptake of transferrinbound iron, and may be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Table 1 lists the 28 analytes measured in this study on the roche cobas 6000 analyzer, along with the assay limit of detection lod, as specified in the manufacturers package insert. Soluble transferrin receptor an overview sciencedirect topics. Using soluble transferrin receptor and taking inflammation. A doubleblind, placebocontrolled study was conducted. Soluble transferrin receptor levels may be helpful in distinguishing iron deficiency from anemia of chronic inflammation in cf because, unlike serum ferritin and transferrin, soluble transferrin receptor does not appear to be an acutephase reactant. The number of stfr reflects the cellular requirements for iron, and varies with the function and the morphological development of each cell type. The existence of a receptor for transferrin iron uptake had been recognized over half a century back. Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apo transferrin is released again 41. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia. Soluble transferrin receptor article about soluble.
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